| 质粒名称: | pCS2 Notch1 Full Length 6MT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41728 |
| 存储实验室: | Raphael Kopan |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2+MT |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4352 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Notch-1 Full Length |
| 物种来源: | M. musculus (mouse) |
| 基因又名: | Notch-1;Notch1;N1 |
| 基因突变: | L1997V; Contains murine Notch-1 protein up to amino acid 2184 |
| GenBank编号 | AAM28905.1 AF508809_1 |
| 标签/融合蛋白: |
6xMyc (C terminal on insert)
|
| 克隆方法: | 未知 |
| 5' 测序引物 | Sp6 |
| 3' 测序引物 | EBV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2 Notch1 Full Length-6MT was a gift from Raphael Kopan (爱迪基因 plasmid # 41728) |
| 参考文献部分: | Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain. Schroeter EH, Kisslinger JA, Kopan R. Nature. 1998 May 28;393(6683):382-6. 10.1038/30756 PubMed 9620803 |
买家导航
pCS2 Notch1 Full Length-6MT载体质粒基本信息
pCS2 Notch1 Full Length-6MT质粒图谱载体图谱和pCS2 Notch1 Full Length-6MT载体序列质粒序列多克隆位点信息
pCS2 Notch1 Full Length-6MT质粒载体简介
pCS2 Notch1 Full Length-6MT质粒序列载体序列
LOCUS pCS2 Notch1 Full Length-6MT 11042 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCS2 Notch1 Full Length-6MT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11042)
AUTHORS Schroeter EH, Kisslinger JA, Kopan R
TITLE Notch-1 signalling requires ligand-induced proteolytic release of
intracellular domain.
JOURNAL Nature. 1998 May 28;393(6683):382-6.
PUBMED 9620803
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2 Notch1 Full Length 6MT|
FEATURES Location/Qualifiers
source 1..11042
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(21..998)
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
rep_origin 1076..1531
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1163..1182)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1373..1394
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 1557..1661
/gene="bla"
/label=AmpR promoter
CDS 1662..2522
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1880..1899)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2693..3281
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3182..3201
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3435..3452
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3569..3590
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3605..3635
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3643..3659
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3648..3670
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3667..3683
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3667..3683
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 3702..3722
/label=T3
/note="T3 promoter, forward primer"
promoter 3704..3722
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 3835..3969
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3872..3891)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3926..3945
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 3974..3991
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(4019..4048)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4082..4111)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4121..4150)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4160..4189)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4199..4228)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(4238..4267)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
primer_bind complement(4281..4297)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4282..4298)
/label=pBluescriptKS
/note="For pBluescript vector"
regulatory 9018..9027
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 10287..10296
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 10619..10628
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(11010..11028)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(11011..11028)
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 cgtccccagg cagaatggcg gttccctaaa cgagcattgc ttatatagac ctcccattag
61 gcacgcctac cgcccattta cgtcaatgga acgcccattt gcgtcattgc ccctccccat
121 tgacgtcaat ggggatgtac ttggcagcca tcgcgggcca tttaccgcca ttgacgtcaa
181 tgggagtact gccaatgtac cctggcgtac ttccaatagt aatgtacttg ccaagttact
241 attaatagat attgatgtac tgccaagtgg gccatttacc gtcattgacg tcaatagggg
301 gcgtgagaac ggatatgaat gggcaatgag ccatcccatt gacgtcaatg gtgggtggtc
361 ctattgacgt caatgggcat tgagccaggc gggccattta ccgtaattga cgtcaatggg
421 ggaggcgcca tatacgtcaa taggaccgcc catatgacgt caataggaaa gaccatatat
481 agagaccatt gacgtcaatg ggggagtggc tatgggcggt attaggaagc cccatatatg
541 gtatatggga ccgcccattg ggaggggcta tctacgtcaa taggaaaacc catatatgga
601 atactatatg gcatagggcc aatacatagt attgaacctg gccaatagcc atattggcat
661 agggccatat tggatattgc ctatatattg atcctggcat atagccaata tggccgccat
721 tattggcacc atgccaattc aatatggcgg acttggcaca gggccaagac ccctcctcaa
781 acttggcacc gtgccaagta gacccctccc cagttggcac agtgccaggt ccgccatatt
841 gaattggcat ggtgccaagt ccgccatatt gaattggctg gggtccagat ccaccatatt
901 gaattggcac gagtccatat acgccatatt gaattggcac aggtccagat ccaccatatt
961 gaattggcat gagtccatat acgccatatt gaattggcgg tcgactggcg taatagcgaa
1021 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
1081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
1141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
1201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
1261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
1321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
1381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
1441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
1501 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
1561 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
1621 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
1681 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
1741 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
1801 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
1861 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
1921 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
1981 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
2041 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
2101 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
2161 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
2221 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
2281 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
2341 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
2401 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
2461 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
2521 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
2581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
2641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
2701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
2761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
2821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
2881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
2941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
3001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
3061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
3121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
3181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
3241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
3301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
3361 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
3421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
3481 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
3541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
3601 aggctttaca ctttatgctc ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
3661 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
3721 gggaacaaaa gctgggtacc gggcccaatg cattggcgcc gcggccgcga attaaaaaac
3781 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
3841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
3961 gtctggatct acgtaatacg actcactata gttctagagg ctcgagaggc cttgaattca
4021 agtcctcttc agaaatgagc ttttgctcca tggtgaggtc gcccaagctc tccatttcat
4081 tcaagtcctc ttcagaaatg agcttttgct ccatttcatt caagtcctct tcagaaatga
4141 gcttttgctc catttcattc aagtcctctt cagaaatgag cttttgctcc atttcattca
4201 agtcctcttc agaaatgagc ttttgctcca tttcattcaa gtcctcttca gaaatgagct
4261 tttgctccat agctttaaat cgataccgtc gacctcgatg acgcgtcatc gagctgtcca
4321 acaggcagcc cttgccatcc tgggacttct tcctccgtgc cttgaggtcc ttagcttcct
4381 tgctaccaca agccagccct ttggtgctgg gcttgcgggc cttcttgccc tgtgtggcag
4441 acttgagatt gcccaggtag ccattgggcg agcagagtgt gggagacaga gtgggtgtgc
4501 cacccagggc agtgccatgc agctgtgggc tgcgcaccag gttgtactca tccaaaagcc
4561 gcacgatatc gtggtgcata cgctcctgtg cgatgtcccg cggcaatcgg tccatgtgat
4621 ccgtgatgtc ccggttggca aagtggtcca gcaacacttt ggcagtctca tagctgccct
4681 cacgggcggc caggaacagg ggagtctcct ccttgttgtt ctgcatgtcc ttgttggctc
4741 cgttcttcag gagcacaaca gcagcatcca cattgttcac cgcggccgcc caatgcaaag
4801 ccgacttgcc taggtcatcc acggcattga cgtcagcatg tgagttgatg aggtcctcca
4861 gcatgccctc cacggccagg cgcgcagcca ggatcagtgg agttgtgcca tcatgcattc
4921 gggcatccag atctgtggcc ctgttccgga gcaggatctg gaagacaccc tgagcatctg
4981 cagaaactgc tgcatgtaac ggagtacggc ccatgttgtc ctggatgttg gcatctgcac
5041 tggcctccag caagcgcttt gcagcatctg aacgagagta tcgggcagcc aagtgcaagg
5101 cggtctcccc ggtgcggtct gtctggttgt gcaagctggc gccctggtag atgaagtcag
5161 agatgacagc aggtgcatct tcttcttctt cactgttgcc tgtctcaagg ccccctccac
5221 tgcaggaggc aatcatgagg ggtgtgaagc catctggtcc tcgaacattg acatccatgc
5281 agtcagcatc cacctccccc tgaggcggtg ttggggccat ggcagacatg cgcaggtcag
5341 cagcgtccag gtgctgctgg gtccactgtc tgtggtcagt ctgatcactc aggtcaggga
5401 gaactactgg ctcctcaaac cggaacttct tggtctccag gtcttcgtct ccccactcgt
5461 tctgattgtc gtccatcaga gcaccatctg aggcattctt caggggcttg aggccgactg
5521 agtcctcgcc gaggggctct ctccgcttct tcttgctggc ctctgacact ttgaaaccct
5581 cagggaacca gagctggcca tgctgccgcc ggcgcttgcg ggacagcagc accccacagc
5641 ccacaaagaa caggagcacg aaggcggccg ctgccacgta catgaggtgc agctgcgagg
5701 gcagcggagg ctccaccggc tcactcttca cggcctcaat cttgtaagga atattgaggc
5761 tgccaagtga cgcaagagca cctaggaagg cagccacatc ggtggcactc tggaagcact
5821 gcgaggatga ctgcacacat tgccggttgt cgatctccag gtagacaatg gagccacgga
5881 tgtccatggg gtccagctcc ctgcgctggc gcccaccact ggtaccagga agcagtgaag
5941 aggtggccca acccactgta gagcgcttga ttgggtgctt gcgcagctct tcctcgtggc
6001 catagtacgg gaagatcatc tgctggcctt gcgcatcacg cttgaagacc acgttggtgt
6061 gcagcacgtg gctgagctcc cgcagaaagt ggaaggagtt gttccgtagc tggtcgggtg
6121 gaagcagcac caccagcacc agggtgccgg ctgccagccg ctcgggtaca tgctcagcac
6181 agtctaggcc atcccactca cattcggcac tgttacagcc ctggtcgcag tggccatcac
6241 tgaagtggtc cttgcagtac tggtcataca gggggttgca ctgtccctcg gtgagctggc
6301 agtcgaagcc atcaaagagg cagccggccg agttgcactg gctgtcacag tggccgtcgc
6361 taaaatactt ccagcactgt agagactgcg tgcagttctt ccaggggtca ttgaagttga
6421 gggagcagtc gccaccatcc cagccacatg cgtgattatt acactgcagg ttgcagacct
6481 tattgcctgc atccacctgg cactcaggca gctcacaggc ctcctcaatc tgcggtgggg
6541 gaatgtcgcg cccagcgcca cctgtgaagc tgtagtccag gatgtggcac agtagcccgt
6601 tgaatttggc agggcataga cagcggtaga aagggttctc ggatgtgggc tcacaggtgc
6661 cctgattgta gcaggggttg ctacccacac aggggctgct ggctgggaac tggcactcag
6721 ggccggtgaa ggatcccagg cataggcagg tgggactacg tgggcccgag atgcatgtac
6781 caccgttgag gcagcgtaag ctgccacaag tgcgggcatc attctcacat gtggcaccct
6841 cgaagcccgc agggcaccta cagatgaatc cacgggcggt gttggaggcc acggcacaga
6901 cacccccatt cttgcaaggt ttgcccctgc agccattgat gactgactca cagcggcgtc
6961 cagtgtggcc agcccggcac tcgcagtgga agtcattaac acgctgcaca cagttctggg
7021 tgccacgtgg gtcacagggg ttggagagac attcattgac atcaccctca caccgctccc
7081 cgacgaagcc tggtgggcag gtgcaggtat agccacccac ctggtccaca caggtgccat
7141 tgttgaagca cttggggctt cgggaggcag ggtcaagggg gggatggcag tcatcaacat
7201 tgatctcaca gtgtacaccc tgtgtccccc gggggcagga acacttgtag gagttggtca
7261 gatcaatgca ggtaccccca ttctggcagg gctgggacag gcactcgttg atctcctctg
7321 agcagttaga cccatggtag ccagccacac acttgcagga aaagccgccg agatagtcag
7381 tgcaggtagc tccattctgg caggggttag gtgagcactc gtccacctcg tcctcacagt
7441 agctgcccgt gtagcctgcc tggcagtggc agtaatgttt atctccctca tccacacaga
7501 gccctccatg ctggcacagg agagtgacgt caatgcctcg cttctgtgca gccacctcac
7561 aggacacact gagcacgtcg cagttgacgc cagtccagcc gctgcggcac tcacagtggt
7621 actgcgtgtt ggtctgccag cacctgccac cattcttgca gggagccgag tcgcaccagc
7681 gcacaaggtt ctggcagttg agaccagtgt agccctgtgg gcaggtacac ttataagtac
7741 catagctgtc ttggcaggta ccaccgtgca gacagggccg tgaatcacac tcattgacat
7801 catactgaca gtagctgccc gtgaagccag gtggacacag acaggtgaag gagttgatac
7861 catccacaca ggtaccacca ttgaagcagg agctctcagt acagtcaggt gtgttgttct
7921 cgcagtggat gccattgaag cccacggggc aggtacatgt gtagctgtcc acacagtcag
7981 tgcaattggc accattttgg cagggattgc tggcacattc attgatgtcc tcctcacaga
8041 aggcaccctg gaagccgggc aggcagtcgc agaaggctgt gttgatgcca tcggtgcagg
8101 aacccccatt gtgacacggg ttggggcggc agtcatcgat gtcactctca cagttgcgac
8161 ctgtatagcc ggcctggcag aggcagcggt agctgccatt ggtgttctgg caggaggccc
8221 catggcgaca tgggcttttc acacactcat tgatgtcaac ctcgcaggtt tgaccttgcc
8281 agcctgtggg acagacacag gaaaaactct catagtcttc agactccttg cataccccgc
8341 tgtttttgca ggggctggta gcacatgggg ccaacaccac ctcacacgtg gctcctgtat
8401 atggcagagg acagttgcac ttgtatccag cgacatcatc aatgcaggtc ccctggttca
8461 ggcaggggtt ggaggcacat tcgttgatgt tggtctggca attagggcca ctgaagcctt
8521 ctcggcaggt gcatacgtag ccactggtca tgtccttgca ggtgccaccg ttgacacaag
8581 ggttggactc acactcgttg ttgttgatgt cacagtttgt tccactccac ccaggggcac
8641 agtcacactt gtacccattg aggccatccc ggcaagctcc gtggatgcag gggttactgt
8701 tgcactcgtt gacctcggac aggcacgtgg ggtcatggta gccctcgggg cagcggcaag
8761 tgaagcccgc gatgccatcc tcacaagtgc ccccgttgtg gcaggggctg cccgcacatt
8821 cgtcaatgtt gacgttacac atgcttcctg tgtagcctgg ttcacaggca cattcgtagc
8881 catcaatctt gtccagacag gtgccagagt cacaggggtt gctggcgcag tcatccaggt
8941 tgatctcaca gttgggccct gtggttccct tgaggcataa gcagaggtag gagttgtcac
9001 ggtcctggca ggtgccccca tggcggcacg gttggctgtg gcactcattg atgttggtct
9061 cacagtgatg gcctgtgtag cctggctggc acaggcaggt aaaggtggcc acaccatcct
9121 tacaggaacc atagtggcag gggtcagggt cacactcgtc aatgtccact tcgcagtggg
9181 tccctgtgta accttctgta cacacgcagg tataggtgtt gggcccatcc aggcacttgg
9241 caccgttctt gcatggtgtg ctggcacact catccacatc atactggcac aggtgcccgt
9301 tgaagccttt ggggcactga cattggaact cattgatctt gtccatgcag tggccattgt
9361 gcagacaggg gctgctggcg cactcatccg tgttgatttc acagtataca ccttcataac
9421 ctggcataca tatgcattgg aactccccaa tctggtccag gcaagtggca tcattctgac
9481 atgggttgga gatgcactca ttaacatcaa tctcacagcg gggtcccgtg tagccctgta
9541 gacactggca ctcaaaagaa cccagtgtgt tgaggcattt gcctgcgtgc tcacaagggt
9601 tggcacccag agcacactca tccacgtcct ggctgcaggc tggccctgtg taccccgagg
9661 ggcaggtgca gatggctttg ccgttgacag ggttggtgtc acagttggag ccctcgttgc
9721 aggggttgct gatgcacgca tcgttgaggt ggcacagcag acctgtgcgc ccatgcggac
9781 attcgcagta gaaggaagcc acacggtcgt ggcaagtggc accctggaaa caggcggcac
9841 tggcacagtc atcaatgttc tcactgcagt cctcgccagt ccacccattg acacacacac
9901 agttgtagcc gccgtgtgtg ttgtggcagg ttccgccatt ctggcaggca ttgggcatga
9961 gctgacattc gtccacatcc tctgtacagt actgacccgt ccactccggt gggcagcggc
10021 aattgtaggt attcacgccg tccacacagg cacccccatt cttgcagttg tttcctggac
10081 agtcatccac attttcttca cagttctgtc cagcaaaacc tggcaagcag gcacactcgt
10141 gggtggtgtc ccctgtagga cggcaggtgc ctccattctg gcagggtgag gggctgcagg
10201 gcacataggg cagttcacag tggggaccag tatgggtggc acggcaggca cagcgatagg
10261 agccgatctc attgtggcag gtgcctccat ggcggcacag cccagggttc tggctgcact
10321 cattaacatc ttgcctgcag gtggggccat ggaagccagg cgggcagcga cagatgtatg
10381 aagactcaaa gggcaggcac tggccaccat tggcacaggg gttggaggca caggggtcag
10441 cctgctgaca tgattttcct gaccaccctg gtgggcagcg gcacttgtac tctgtgagag
10501 tgagcaggtc acaggtgccc ccattgcggc aggggttggc caggcaggcg ttgtccagag
10561 gtgtcaggca gaggggccca gagaaaccca ggggacagct gcaggcataa tccacagtgc
10621 caccatggtc cacaacgtgg cacgttccag cattcttaca cggtgtgctg aggcaaggat
10681 tggagtcctg gcatcgttgg cccacaaagg ctccgctgca gacacaggct tcagtgccgc
10741 tggccacttc gcacctacct ccattcaggc aggtcccact tggctgggag catctcaagc
10801 ctcttgcggc gagcgcgggc agcagcgtta ggcagagcaa gggcgtcagg agccgtggca
10861 tgcctgctcg ccagctgccc gcagcccggg cggcggcctc ttggggtggc cggcggggat
10921 cggacgttga cgcacaccac cggagcccag cgccgcccac ccggaattcg aatcgatggg
10981 atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc
11041 ga
//
